pyrR [2020-09-10 09:29:13]

transcriptional antiterminator of the pyr operon

locus: BSU_15470

pI: 4.99

mw: 20.12 kDa

protein length: 181 aa

gene length: 546 bp

function: regulation of pyrimidine biosynthesis

product: transcriptional antiterminator with minor uracil phosphoribosyltransferase activity

essential: no

ec: 2.4.2.9

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]

Gene

Coordinates: 1,618,304 1,618,849

The protein

Catalyzed reaction/ biological activity

• diphosphate + UMP --> 5-phospho-α-D-ribose 1-diphosphate + uracil (according to UniProt)

Protein family

• [SW|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

Effectors of protein activity

• UMP and UTP stimulate the RNA-binding activity of PyrR, resulting of transcription termination of the [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE] operon [pubmed|31100987]

Structure

• [PDB|1A3C] (dimeric form)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000061 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
  description: [Pubmed|8206849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1709162], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR]: termination/ antitermination, via [SW|RNA switch], in [regulon|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR regulon]

  regulation

  • induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]

• genes: [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
  description: [Pubmed|22383849]

• genes: [gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA
• BKK15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA

References

Reviews

• 28031352,31100987

Original publications

• 8935652,8206849,15716449,11726695,9488732,9551555,12896995,9932459,8955403,8824632,12482852,1709162,7798145,11948166,8759868,7868607,9488732,17322189